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AOX1 DNA segment and the transcription termination-polyadenylation sequence from the AOX1 gene (TT), into the chro mosome.
AOX1 segment, the transcription termina tion-polyadenylation sequence from the AOX1 gene (TT), and a functional HIS4 gene.
The multiple cloning site (MCS) and selectable marker gene (SMG) are under the control of eukaryotic pro moter (p), polyadenylation (pa), and termination of transcription (TT) sequences.
transcription (TT) sequences.
Both expression vectors carry origins of replication for E. coli (oriE) and mammalian cells (orieuk), a selectable marker (Ampr) for selecting transformed E. coli, and a select able marker gene (SMG) that is under the control of eukary otic promoter (p), polyadenylation (pa), and termination of transcription (TT) sequences.
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